LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1955..2051) /label=attL2 /note="attL2 (c)" misc_signal complement(2066..2085) /note="T7 Promoter/priming site (c)" primer_bind 2093..2109 /note="M13 Reverse priming site" gene 2222..3031 /note="Kanamycin resistance gene" rep_origin 3152..3825 /note="pUC origin" vector join(1963..3828,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1948 /organism="synthetic construct" /mol_type="other DNA" /db_xref="taxon:32630" /clone="MGC:193185 IMAGE:100064006" /clone_lib="NIH_MGC_435" /lab_host="DH10B" /focus /note="Vector: pENTR223.1 with stop codon" source 692..1948 /organism="Homo sapiens" /mol_type="other DNA" /db_xref="taxon:9606" gene 692..1948 /gene="GCNT4" /gene_synonym=C2GNT3 /db_xref="GeneID:51301" /db_xref="HGNC:17973" CDS 692..1948 /dnas_title="glucosaminyl (N-acetyl) transferase 4, core 2 (beta-1,6-N-acetylglucosaminyltransferase)" /gene="GCNT4" /gene_synonym=C2GNT3 /codon_start=1 /product="glucosaminyl (N-acetyl) transferase 4, core 2 (beta-1,6-N-acetylglucosaminyltransferase)" /protein_id="AAI60070.1" /db_xref="GI:182888315" /db_xref="GeneID:51301" /db_xref="HGNC:17973" /translation="MKIFKCYFKHTLQQKVFILFLTLWLLSLLKLLNVRRLFPQKDIY LVEYSLSTSPFVRNRYTHVKDEVRYEVNCSGIYEQEPLEIGKSLEIRRRDIIDLEDDD VVAMTSDCDIYQTLRGYAQKLVSKEEKSFPIAYSLVVHKDAIMVERLIHAIYNQHNIY CIHYDRKAPDTFKVAMNNLAKCFSNIFIASKLEAVEYAHISRLQADLNCLSDLLKSSI QWKYVINLCGQDFPLKSNFELVSELKKLNGANMLETVKPPNSKLERFTYHHELRRVPY EYVKLPIRTNISKEAPPHNIQIFVGSAYFVLSQAFVKYIFNNSIVQDFFAWSKDTYSP DEHFWATLIRVPGIPGEISRSAQDVSDLQSKTRLVKWNYYEGFFYPSCTGSHLRSVCI YGAAELRWLIKDGHWFANKFDSKVDPILIKCLAEKLEEQQRDWITLPSEKLFMDRNLT TTS" misc_feature 692..1948 /note="GCNT4 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cagacgactc tttccgcaaa aagacattta 721 cttggttgag tactccctaa gtacctcgcc ttttgtaaga aacagataca ctcatgttaa 781 ggatgaagtc aggtatgaag ttaactgttc gggtatctat gaacaggagc ctttggaaat 841 tggaaagagt ctggaaataa gaagaaggga catcattgac ttggaggatg atgatgttgt 901 ggcaatgacc agtgattgtg acatttatca gactctaaga ggttatgctc aaaagcttgt 961 ctcaaaggag gagaaaagct tcccaatagc ctattctttg gttgtccaca aagatgcaat 1021 tatggttgaa aggcttatcc atgctatata caaccagcac aatatttact gcatccatta 1081 tgatcgtaag gcacctgata ccttcaaagt tgccatgaac aatttagcta agtgcttctc 1141 caatattttc attgcttcca aattagaggc tgtggaatat gcccacattt ccagactcca 1201 ggctgattta aattgcttgt cggaccttct gaagtcttca atccagtgga aatatgttat 1261 caacttgtgt gggcaagatt ttcccctgaa gtcaaatttt gaattggtgt cagagttgaa 1321 aaaactcaat ggagcaaata tgttggagac ggtgaaaccc ccaaacagta aattggaaag 1381 attcacttac catcatgaac ttagacgggt gccttatgaa tatgtgaagc taccaataag 1441 gacaaacatc tccaaggaag caccccccca taacattcag atatttgttg gcagtgctta 1501 ttttgtttta agtcaagcat ttgttaaata tattttcaac aactccatcg ttcaagactt 1561 ttttgcctgg tctaaagaca catactctcc tgatgagcac ttttgggcta ccttgattcg 1621 ggttccagga atacctgggg agatttccag atcagcccag gatgtgtctg atctgcagag 1681 taagactcgc cttgtcaagt ggaattacta tgaaggcttt ttctatccca gttgtactgg 1741 atctcacctt cgaagcgtgt gtatttatgg agctgcagaa ttaaggtggc ttatcaaaga 1801 tggacattgg tttgctaata aatttgattc taaggtggac cctatcttga ttaaatgctt 1861 ggcagaaaag cttgaagaac agcagagaga ctggatcact ttgccctcag aaaagttatt 1921 tatggataga aatctcacta ccacatcaTA GGACCCAGCT TTCTTGTACA AAGTTGGCAT 1981 TATAAGAAAG CATTGCTTAT CAATTTGTTG CAACGAACAG GTCACTATCA GTCAAAATAA 2041 AATCATTATT TGCCATCCAG CTGATATCCC CTATAGTGAG TCGTATTACA TGGTCATAGC 2101 TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA AAATCTCTGA TGTTACATTG CACAAGATAA 2161 AATAATATCA TCATGAACAA TAAAACTGTC TGCTTACATA AACAGTAATA CAAGGGGTGT 2221 TATGAGCCAT ATTCAACGGG AAACGTCGAG GCCGCGATTA AATTCCAACA TGGATGCTGA 2281 TTTATATGGG TATAAATGGG CTCGCGATAA TGTCGGGCAA TCAGGTGCGA CAATCTATCG 2341 CTTGTATGGG AAGCCCGATG CGCCAGAGTT GTTTCTGAAA CATGGCAAAG GTAGCGTTGC 2401 CAATGATGTT ACAGATGAGA TGGTCAGACT AAACTGGCTG ACGGAATTTA TGCCTCTTCC 2461 GACCATCAAG CATTTTATCC GTACTCCTGA TGATGCATGG TTACTCACCA CTGCGATCCC 2521 CGGAAAAACA GCATTCCAGG TATTAGAAGA ATATCCTGAT TCAGGTGAAA ATATTGTTGA 2581 TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA TTCGATTCCT GTTTGTAATT GTCCTTTTAA 2641 CAGCGATCGC GTATTTCGTC TCGCTCAGGC GCAATCACGA ATGAATAACG GTTTGGTTGA 2701 TGCGAGTGAT TTTGATGACG AGCGTAATGG CTGGCCTGTT GAACAAGTCT GGAAAGAAAT 2761 GCATAAACTT TTGCCATTCT CACCGGATTC AGTCGTCACT CATGGTGATT TCTCACTTGA 2821 TAACCTTATT TTTGACGAGG GGAAATTAAT AGGTTGTATT GATGTTGGAC GAGTCGGAAT 2881 CGCAGACCGA TACCAGGATC TTGCCATCCT ATGGAACTGC CTCGGTGAGT TTTCTCCTTC 2941 ATTACAGAAA CGGCTTTTTC AAAAATATGG TATTGATAAT CCTGATATGA ATAAATTGCA 3001 GTTTCATTTG ATGCTCGATG AGTTTTTCTA ATCAGAATTG GTTAATTGGT TGTAACACTG 3061 GCAGAGCATT ACGCTGACTT GACGGGACGG CGCAAGCTCA TGACCAAAAT CCCTTAACGT 3121 GAGTTACGCG TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG 3181 AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC 3241 GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG 3301 CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA 3361 GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC 3421 CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC 3481 GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA 3541 CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG 3601 AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT 3661 TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA 3721 GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC 3781 GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTT //